SELECTED PUBLICATIONS      ALL PUBS link                                                                                                                  Eleanore T. Wurtzel

NOTE: journal names or "PDF link" link to the cited paper

Wurtzel, E. T., Vickers, C.E., Hanson, A.D., Millar. A.H., Cooper, M., Voss-Fels, K.P., Nikel, P.I., and Erb, T.J. (2019) Revolutionizing agriculture with synthetic biology. Nature Plants doi:10.1038/s41477-019-0539-0 (free link:


Wurtzel, E. T. and Beltrán, J. (2019) Improved expression and purification of the carotenoid biosynthetic enzyme Z-ISO in Plant and Food Carotenoids: Methods and Protocols. Methods in Molecular Biology (Concepción M.R., Welsch R., eds) 2083: 53-61 Springer-Nature, New York, USA DOI:


Shumskaya, M., Quinlan, R.F., Wurtzel, E. T. (2019) Elucidating carotenoid biosynthetic enzyme localization and interactions using fluorescent microscopy in Plant and Food Carotenoids: Methods and Protocols. Methods in Molecular Biology (Concepción M.R., Welsch R., eds) 2083:223-234 Springer-Nature, New York, USA DOI:


Wurtzel, E. T. (2018) Changing form and function through carotenoids and synthetic biology. Plant Physiology (Synthetic Biology Focus Issue) DOI:

first author profile (


Timlin, J. A., Collins, A. M., Beechem T.A., Shumskaya, M., Wurtzel, E. T. (2017) Localizing and quantifying carotenoids in intact cells and tissues. IN Cvetkovic D.J., Nikolic G.S. (eds) Carotenoids Chapter 3, InTech, Rijeka


Wurtzel, E. T. and Kutchan, T. M. (2016) Plant metabolism, the diverse chemistry set of the future. Science 353: 1232-1236. Invited review in special issue on “Translational Plant Science"


Beltrán, J., Kloss, B., Hosler, J.P., Geng, J., Liu, A., Modi, A., Dawson, J.H., Sono, M., Shumskaya, M., Ampomah-Dwamena, C., Love, J.D., Wurtzel, E.T (2015) Control of carotenoid biosynthesis through a heme-based cis-trans isomerase. Nature Chemical Biology 11(8):598-605 (June 15, 2015 advance online, doi:10.1038/nchembio.1840).


Ampomah-Dwamena, C., Driedonks, N., Lewis, D., Shumskaya, M., Chen, X., Wurtzel, E.T., Espley, R.V., Allan, A.C. (2015) The Phytoene synthase gene family of apple (Malus x domestica) and its role in controlling fruit carotenoid content.  BMC Plant Biology 15:185 (doi:10.1186/s12870-015-0573-7)

Moise, A., Al-Babili, S.,  and Wurtzel, E.T. (2014) Mechanistic aspects of carotenoid synthesis. Chemical Reviews 114:164-193 (Special issue on Chemistry and Biology of Retinoids and Carotenoids, guest editor: Kris Palczewski) PMID: 24175570 .

Shumskaya, M. and Wurtzel, E.T. (2013) The carotenoid biosynthetic pathway: thinking in all dimensions. Plant Science (208): 58-63 (

Tzfadia, O., Amar, D., Bradbury, L.M.T., Wurtzel, E.T., and Shamir, R. (2012) The MORPH algorithm: ranking candidate genes for membership in Arabidopsis thaliana and Solanum lycopersicum pathways. The Plant Cell 24 (11): 4389-4406

Shumskaya, M., Bradbury, L.M.T., Monaco, R.R., and Wurtzel, E.T. (2012) Plastid localization of the key carotenoid enzyme phytoene synthase is altered by isozyme, allelic variation and activity. The Plant Cell 24 (9): 3725-3741.

Bradbury, L.M.T.Shumskaya, M., Tzfadia, O.,  Wu, S.-B., Kennelly, E., and Wurtzel, E.T. (2012) Lycopene cyclase paralog CruP protects against reactive oxygen species in oxygenic photosynthetic organisms. Proc. Natl. Acad. Sci., USA. 109 (27) E1888–E1897 published online June 15, 2012, doi: 10.1073/pnas.1206002109

Quinlan, R.F. Shumskaya, M., Bradbury, Beltrán, J., L.M.T., Ma, C., E.J. Kennelly, and Wurtzel, E.T. (2012) Synergistic interactions between carotene ring hydroxylases drive lutein formation in plant carotenoid biosynthesis. Plant Physiology 160:204-214.

Wurtzel ET, Cuttriss A and Vallabhaneni R (2012) Maize provitamin A carotenoids, current resources, and future metabolic engineering challenges. Frontiers in Plant Science 3:29. doi: 10.3389/fpls.2012.00029

Joët, T, Wurtzel, E.T., Matsuda, F., Saito, K., Dussert, S. (2012) Coupled transcript-metabolite profiling: towards system biology approaches to unravel regulation of seed secondary metabolism. IN SEED DEVELOPMENT: OMICS Technologies toward Improvement of Seed Quality and Crop Yield, (Ganesh K. Agrawal, ed.) SPRINGER (The Netherlands), pp. 367-385. PDF link

Cuttriss, A.J., Cazzonelli, C.I., Wurtzel, E.T. and Pogson, B.J. (2011) Carotenoids. IN Rébeillé, F. and Douce, R. (eds) Biosynthesis of Vitamins in Plants, Advances in Botanical Research, vol. 58, p. 1-36, Elsevier, Amsterdam, The Netherlands PDF link
Meier, S., Tzfadia, O., Vallabhaneni, R., Gehring, C. and  Wurtzel, E.T. (2011) A transcriptional analysis of carotenoid, chlorophyll and plastidial isoprenoid biosynthesis genes during development and osmotic stress responses in Arabidopsis thaliana. BMC Systems Biology, 5:77
Landrum JT, Wang X-D, Wurtzel ET (2010) Guest editors' introduction. Archives of Biochemistry and Biophysics (Carotenoids Highlight edition) 504: 1-2.
Vallabhaneni, R., Bradbury, L., and Wurtzel, E. T., (2010) The carotenoid dioxygenase gene family in maize, sorghum, and rice, Archives in Biochemistry and Biophysics (Carotenoids Highlight edition) 504: 104–111. Published online 7/27/2010; doi:10.1016/  PDF supp data

Vallabhaneni, R. and Wurtzel, E. T., (2010) From epoxycarotenoids to ABA: the role of ABA 8’-hydroxylases in drought-stressed maize roots, Archives in Biochemistry and Biophysics (Carotenoids Highlight edition) 504: 112–117. Published online 7/14/2010; 10.1016/ PDF supp data

Chen, Yu, Li, F., and Wurtzel, E.T. (2010) Isolation and characterization of the Z-ISO gene encoding a missing component of carotenoid biosynthesis in plants. Plant Physiology 153: 66-79 Published online 3/24/10, 10.1104/pp.110.153916. 
Vallabhaneni, R., Gallagher, C.E., Licciardello, N., Cuttriss, A.J., Quinlan, R.F., and Wurtzel, E.T. (2009) Metabolite sorting of a germplasm collection reveals the Hydroxylase3 locus as a new target for maize provitamin A biofortification. Plant Physiology 151 (3): 1635-1645. Published on September 18, 2009; 10.1104/pp.109.145177.
Vallabhaneni, R. and Wurtzel, E.T. (2009) Timing and biosynthetic potential for provitamin A accumulation in maize. Plant Physiology 150(2):562-572 supplementary data.

 (combined PDF). Published on April 3, 2009; 10.1104/pp.109.137042.

Li, F., Tzfadia, O., and Wurtzel, E.T. (2009) The Phytoene Synthase gene family in the Grasses: Subfunctionalization provides tissue-specific control of carotenogenesis. Plant Signaling and Behavior 4(3): 208-211 (March 2009).
Li, F., Vallabhaneni, R., Yu, J., Rocheford, T., and Wurtzel, E.T. (2008) The maize phytoene synthase gene family: overlapping roles for carotenogenesis in endosperm, photomorphogenesis, and thermal stress-tolerance. Plant Physiology 147:1334-1346 (Published on May 28, 2008; 10.1104/pp.108.122119) supplementary material online.
Harjes, C.E., Rocheford, T., Bai, L., Brutnell, T., Bermudez-Kandianis, C., Sowinski, S.G., Stapleton, A.E., Vallabhaneni, R., Williams, M., Wurtzel, E.T., Yan, J., Buckler, E.S. (2008) Natural genetic variation in Lycopene Epsilon Cyclase tapped for maize biofortification. Science 319:330-333; supplementary material online.
Li, F., Vallabhaneni, R., and Wurtzel, E.T. (2008) PSY3, a new member of the phytoene synthase gene family conserved in the Poaceae and a key regulator of abiotic-stress-induced root carotenogenesis. Plant Physiology 146 (3): 1333-1345 supplementary material online. First published online December 27, 2007; 10.1104/pp.107.111120
Li, F., Murillo, C., and Wurtzel, E.T. (2007) Maize Y9 is essential for 15-cis-zetacarotene isomerization. Plant Physiology 144:1181-1189.  First published online April 13, 2007; 10.1104/pp.107.098996
Quinlan, R.F., Jaradat, T.T., and Wurtzel, E.T. (2007) Escherichia coli as a platform for functional expression of plant P450 carotene hydroxylases. Highlight Edition on Carotenoids, Archives of Biochemistry and Biophysics 458:146-157. (Available online 3 December 2006)
Matthews, P.D. and Wurtzel, E.T. (2007) Biotechnology of food colorant production in Food Colorants: Chemical and Functional Properties. (Carmen Socaciu, Editor) CRC Press. pdf
Cervantes-Cervantes, M., Gallagher, C.E., Zhu, C. and Wurtzel, E.T. (2006) Maize cDNAs expressed in endosperm encode functional farnesyl diphosphate synthase with geranylgeranyl diphosphate synthase activity Plant Physiology 141:220-231.
Wurtzel, E.T. and Grotewold, E. (2006) Plant Metabolic Engineering in The Encyclopedia of Chemical Processing (Sunggyu (K.B.) Lee, Editor), p. 2191-2200, Marcel Dekker, Inc, New York, USA  pdf
Gallagher, C.E., Matthews, P.D., Faqiang Li, and Wurtzel, E.T. (2004) Gene duplication in the carotenoid biosynthetic pathway preceded evolution of the grasses (Poaceae). Plant Physiology 135:1776-1783 rice PSY1 RT-PCR conditions
Wurtzel, E.T. (2004) Genomics, genetics, and biochemistry of maize carotenoid biosynthesis. In Recent Advances in Phytochemistry, vol 38: 85-110 (J. Romeo, editor), Elsevier.   chapter
Wong, J.C., Lambert, R.J., Wurtzel, E.T., and Rocheford, T.R. (2004) QTL and candidate genes phytoene synthase and zetacarotene desaturase associated with the accumulation of carotenoids in  maize. Theoretical and Applied Genetics 108(2):349-359
Matthews, P.D., Luo, R., and Wurtzel, E.T. (2003) Maize phytoene desaturase and zetacarotene desaturase catalyze a poly-Z desaturation pathway: implications for genetic engineering of carotenoid content among cereal crops. Journal of Experimental Botany 54 (391):2215-2230.
Gallagher, C. E.,  Cervantes-Cervantes, M. and  Wurtzel, E. T. (2003) Surrogate biochemistry: use of Escherichia coli to identify plant cDNAs that impact metabolic engineering of carotenoid accumulation Applied Microbiology and Biotechnology 60:713-719.
Wurtzel, E. T. (2001)  Rice Genetics: Engineering Vitamin A in Encyclopedia of  Genetics, Eric Reeve (editor) p. 668-671, Fitzroy Dearborn Publishers, Chicago, IL. PDF link
Wurtzel, E. T., Luo, R., and Yatou, O. (2001) A simple approach to identify the first rice mutants blocked in carotenoid biosynthesis.  Journal of Experimental Botany 52(354): 161-166.
Matthews, P. D. and Wurtzel, E. T. (2000) Metabolic engineering of carotenoid accumulation in Escherichia coli by modulation of the isoprenoid precursor pool with expression of deoxyxylulose phosphate synthase. Applied Microbiology and Biotechnology 53:396-400.
Vigneswaran, A.  and Wurtzel, E. T. (1999) A rice cDNA encoding phytoene desaturase (Accession No. AF049356). (PGR99-131) Plant Physiology 121:311.
Luo, R. and Wurtzel, E. T. (1999) A maize cDNA encoding zeta carotene desaturase (Accession No. AF047490). (PGR99-118) Plant Physiology 120:1206.
Li, Z.-h. and Wurtzel, E.T. (1998) The Ltk gene family encodes novel receptor-like kinases with temporal expression in developing maize endosperm. Plant Mol Biology 37:749-761.
Maluf, M.P., Saab, I.N., Wurtzel, E.T., Sachs, M.M. (1997) The viviparous12 maize mutant is deficient in abscisic acid, carotenoids, and chlorophyll synthesis. J Experimental Botany 48(311): 1259-1268.
Wurtzel, E.T., Valdez, G., and Matthews, P.D. (1997) Variation in expression of carotenoid genes in transformed E. coli strains. Bioresearch Journal 1(4): 1-11.

Wurtzel, E. T. (1997) The vp5 gene. In Mutants of Maize  by M.G. Neuffer, E.H. Coe, S.R. Wessler, Cold Spring Harbor Laboratory Press, New York.  

Buckner, B., Wurtzel, E.T., SanMiguel, P. (1997) The y1 gene. In Mutants of Maize  by M.G. Neuffer, E.H. Coe, S.R. Wessler, Cold Spring Harbor Laboratory Press, New York. 

Gallagher, J.C. and Wurtzel, E.T. (1997) Genetic diversity of phytoplankton: Implications for species concepts and bloom dynamics. pp. 129‑135. In Martins, M.T., Sato, M.I.Z., Tiedje, J.M.,

Hagler, L.C.N., Dobereiner, J., and Sanchez, P.S. (Eds.) Progress in Microbial Ecology, SBM/ICOME, Sao Paulo, Brazil. 

Wurtzel, E.T., Li, Z., Luo, R., Matias, D., Mozoub, D., Matthews, P.D., Upasani, V.,Valdez, G., Yoganathan, A., Yu, J. (1996) Research towards improvement of the pro-vitamin A (carotenoid) content of rice endosperm. International Rice Research Notes 21:2-3.
Li, Z.-h., Matthews, P.D., Burr, B. and Wurtzel, E.T. (1996) Cloning and characterization of a maize cDNA encoding phytoene desaturase, an enzyme of the carotenoid biosynthetic pathway. Plant Mol Biology 30: 269-279.

De Luca P, Moretti, A., Siniscalco-Gigliano, G., Caputo, P., Cozzolino S., Gaudio, L., Stevenson, D.W., Wurtzel, E.T. and Osborne, R. (1995) Molecular systematics of cycads. In: P. Voorster (ed.), Proceedings of the Third International Conference on Cycad Biology. Stellenbosch, South Africa. Pp. 131-137.

Matthews, P.D., and Wurtzel, E.T. (1995) A high throughput microplate format for producing and screening riboprobes from bacterial cells. Biotechniques 18(6):1000-1004.

Stabile, J.E. and Wurtzel, E.T.  (1994) A simple method for simultaneous purification of multiple, large DNA fragments from agarose gels.  Biotechniques 17(4):644-649.

Caputo, P., Marquis C., Stevenson, D. and Wurtzel, E.T. (1993) Molecular biological approaches to cycad systematics, in The Biology, Structure, and Systematics of the Cycadales: Proceedings of the Second International Conference on Cycad Biology, Stevenson DW and Norstog KJ (eds), Palms and Cycad Societies of Australia, Ltd, pp. 213-219.

Wurtzel, E.T. (1992) Use of a Ds chromosome-breaking element to examine maize Vp5 expression. J. Heredity 83: 109-113

Stabile, J., Wurtzel, E.T., and Gallagher, J.  (1992) Comparison of chloroplast DNA and allozyme variation in winter strains of the marine diatom, Skeletonema costatum (Bacillariophyta). Journal of Phycology 28:90-94.

Fontes, E.B.P., Shank, B.B., Wrobel, R.L., Moose S.P., OBrian, G.R.,  Wurtzel, E.T., and Boston, R.B. (1991) Characterization of an immunoglobulin binding protein homolog in the maize floury-2 endosperm mutant. The Plant Cell 3: 483-496.

Lindsay, S.M., Philipp, M., and Wurtzel, E.T. (1991) Can the scanning tunneling microscope sequence DNA?  Genetic Analysis: Techniques and Applications 8:8-13.

Caputo, P., Stevenson, D., and Wurtzel, E.T.  (1991) A phylogenetic analysis of American Zamiaceae (Cycadales) using chloroplast DNA restriction fragment length polymorphisms. Brittonia 43(3):135-145.

Stabile, J., Gallagher, J., and Wurtzel, E.T. (1990) Molecular analysis of intraspecific variation in the marine diatom Skeletonema costatum. Biochemical Systematics and Ecology 18:5-9.                             

Shank, B.B., Bass H.W., Sisco, P.H., Wurtzel, E.T., and Boston, R.B. (1990) Isolation and mapping of a cDNA probe for the b-70 protein. Maize Genetics Coop. Newsl. 64: 97.

Wurtzel, E.T., Burr, F.A., and Burr, B. (1987) DNase I hypersensitivity and expression of the Shrunken-1 gene of maize. Plant Mol. Biology 8: 251-264.

Wurtzel, E.T., Chou, M.-Y., and Inouye, M. (1982) Osmoregulation of gene expression:  I. DNA sequence of the ompR gene of the ompB operon of E. coli and characterization of its gene product. Journal of Biological Chemistry 257:13685-13691.

Mizuno, T., Wurtzel, E.T., and Inouye, M. (1982) Osmoregulation of gene expression:  II. DNA sequence of the envZ gene of the ompB operon of E. coli and characterization of its gene product. Journal of Biological Chemistry 257:13692-13698.  

Mizuno, T., Wurtzel, E.T., and Inouye, M. (1982) Cloning of the regulatory genes (ompR and envZ) for the matrix proteins of the E. coli outer membrane. Journal of Bacteriology 150:1462-1466.

Wurtzel, E.T., Movva, N.R., Ross, F., and Inouye, M. (1981) Two-step cloning of the E. coli regulatory gene ompB, employing phage Mu. Journal of Molecular  and Applied Genetics 1:61-69.

Nakamura, K., Katz-Wurtzel, E.T., Pirtle, R.M., and Inouye, M. (1979)  Restriction enzyme cleavage sites surrounding the structural gene for the lipoprotein of the E. coli outer membrane.  Journal of Bacteriology 138:715-720.

Katz, E., Loring, D., Inouye, M. (1978) Lipoprotein from P. mirabilis.  Journal of Bacteriology 134:674-676.

Movva, N.R., Katz, E., Asdourian, P., Hirota, Y., and Inouye, M. (1978)  Gene dosage effects of the structural gene for a lipoprotein of the E. coli outer membrane. Journal of Bacteriology 133:81-84.






last edited 03/27/2017